Vitamin B12 (Cyanocobalamin)

Vitaminwater-solubleStrong dose evidence

Also known as: cyanocobalamin, vitamin B12, cobalamin, B12

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Cyanocobalamin is the synthetic, highly stable form of vitamin B12 used in most supplements and fortified foods. NIH ODS notes it becomes active after conversion to methylcobalamin and adenosylcobalamin, the two coenzyme forms. Vitamin B12 is needed for red blood cell formation, nerve myelin, and homocysteine metabolism, and oral doses of 1,000 to 2,000 mcg have matched injections in trials on deficient patients, because a small fraction is absorbed passively even without intrinsic factor. No UL exists.

Research summary

Reduced and decyanated in cells to cob(II)alamin, then converted to methylcobalamin (methionine synthase) and adenosylcobalamin (methylmalonyl-CoA mutase).

Dosage

Daily amounts, of compound.

Lowest studiedTypical rangeHighest studiedUpper limit
2 mcg100–1,000 mcg2,000 mcg

Doses are mcg cyanocobalamin. RDA is 2.4 mcg for adults; DV 2.4 mcg. NIH ODS reports no UL because B12 is considered safe even at high doses. A systematic review of randomized trials compared oral 2,000 mcg daily (and 1,000 mcg regimens) with intramuscular B12 in deficient patients and found oral dosing effective. Absorption via intrinsic factor saturates at a few mcg per dose, so high oral doses rely on passive uptake; take without regard to meals. Stable to heat and light better than other cobalamins.

Standardization: ≥96% cyanocobalamin (USP) or 0.1% to 1% trituration

Source material: fermentation (Pseudomonas or Propionibacterium), purified

As supplied: Typically a 1% trituration on a carrier (0.1 mg powder per mcg); 0.1% grades are used for very low doses

Forms of Vitamin B12

FormTypical doseElement %FormatsPrice
Vitamin B12 (Cyanocobalamin)100–1,000 mcgCapsule, Tablet, Powder, Gummy, Liquid, Softgellow
Vitamin B12 (Methylcobalamin)100–1,000 mcgCapsule, Tablet, Powder, Liquid, Gummymid
Vitamin B12 (Adenosylcobalamin)100–1,000 mcgCapsule, Tablet, Powder, Liquidhigh

Format suitability

  • Capsule
  • Powder
  • Tablet
  • Softgel
  • Gummy
  • Liquid

Deep red; tints powders and liquids pink at high inclusion. Most stable B12 for gummies and liquids.

Formulation notes

Pair with folate in any formula above 400 mcg folic acid. Metformin and acid-suppressing drugs lower B12 status. Degraded by vitamin C in solution over time.

Sources

  1. Vitamin B12 — Health Professional Fact SheetNIH ODS
  2. Oral vitamin B12 versus intramuscular vitamin B12 for vitamin B12 deficiency: a systematic review of randomized controlled trials. (Butler CC, 2006)PubMed

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