Folic Acid

Vitaminwater-solubleStrong dose evidence

Also known as: pteroylmonoglutamic acid, folacin, vitamin B9, synthetic folate, PGA

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Folic acid is the fully oxidized, synthetic form of folate used in food fortification and most supplements. It is highly bioavailable (about 85 percent with food, near 100 percent fasting) and is the form with the strongest evidence base for periconceptional neural tube defect risk reduction at 400 mcg per day. It supports DNA synthesis, cell division, and homocysteine metabolism. Labels declare it in mcg DFE, where 1 mcg folic acid equals 1.7 mcg DFE, and a 1,000 mcg UL applies to the synthetic form.

Research summary

Reduced by dihydrofolate reductase to tetrahydrofolate and then to 5-MTHF and 10-formyl-THF, the one-carbon carriers for nucleotide synthesis and homocysteine remethylation.

Dosage

Daily amounts, of compound.

Lowest studiedTypical rangeHighest studiedUpper limit
400 mcg400–800 mcg1,000 mcg1,000 mcg

NIH ODS — UL is 1,000 mcg/day of folic acid from fortified foods and supplements for adults 19+; food folate has no UL

Doses are mcg folic acid. RDA is 400 mcg DFE (600 mcg DFE in pregnancy); DV 400 mcg DFE, so 400 mcg folic acid = 680 mcg DFE (170% DV) and must be labeled as mcg DFE with the folic acid amount in parentheses. NIH ODS reports common adult supplement doses of 400 to 800 mcg folic acid. dose_max_studied is capped at the UL; 2,500 mcg was used in a cardiovascular trial and 4,000 to 5,000 mcg is recommended for women with a prior NTD pregnancy, both under medical supervision. High folic acid can mask the anemia of B12 deficiency, so pair with B12.

Standardization: ≥97% folic acid (USP) or 1% trituration

Source material: synthetic

As supplied: Often used as a 1% trituration on a carrier for premixes (0.1 mg powder per mcg); neat material is used for higher doses. Supplier dependent.

Forms of Folate

FormTypical doseElement %FormatsPrice
Folic Acid400–800 mcgCapsule, Tablet, Powder, Gummy, Liquid, Softgellow
L-5-Methyltetrahydrofolate (L-5-MTHF)400–1,000 mcgCapsule, Tablet, Powder, Gummy, Liquid, Softgelhigh
Folinic Acid (Calcium Folinate)400–800 mcgCapsule, Tablet, Powder, Liquidhigh

Format suitability

  • Capsule
  • Powder
  • Tablet
  • Softgel
  • Gummy
  • Liquid

Yellow-orange; light-sensitive but otherwise stable. Fine in gummies.

Formulation notes

Always pair with vitamin B12 in adult formulas. Interacts with methotrexate, some antiepileptics, and sulfasalazine. Standard prenatal component alongside iron, choline, and DHA.

  • Has an upper limit
  • Notable drug interactions

Sources

  1. Folate — Health Professional Fact SheetNIH ODS
  2. Supplementation with Folic Acid or 5-Methyltetrahydrofolate and Prevention of Neural Tube Defects: An Evidence-Based Narrative Review. (Samaniego-Vaesken ML, 2024)PubMed

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