Folinic Acid (Calcium Folinate)

Vitaminwater-solubleLimited dose evidence

Also known as: calcium folinate, leucovorin, 5-formyltetrahydrofolate, 5-formyl-THF, citrovorum factor

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Folinic acid (5-formyltetrahydrofolate) is a reduced, metabolically active folate supplied as its calcium salt. Unlike folic acid it does not require dihydrofolate reductase, and unlike L-5-MTHF it enters the folate cycle upstream of the MTHFR step, so it can feed both methylation and nucleotide synthesis. Its high-dose calcium salt is the drug leucovorin. As a supplement ingredient it appears in specialty folate, methylation, and neurodevelopment-support formulas, with randomized trials in children using much larger, physician-directed doses.

Research summary

Converted to 5,10-methenyl-THF and then to other tetrahydrofolate cofactors for purine, thymidylate, and methionine synthesis without needing DHFR or MTHFR.

Dosage

Daily amounts, of compound.

Lowest studiedTypical rangeHighest studiedUpper limit
400 mcg400–800 mcg1,000 mcg1,000 mcg

NIH ODS — UL of 1,000 mcg/day is set for folic acid from supplements and fortified foods; applied to this synthetic folate form as a conservative ceiling

Doses are mcg calcium folinate as labeled. Supplement-level amounts of 400 to 800 mcg mirror folic acid practice rather than a dose-finding trial of this form, and the same 400 mcg DFE DV framework applies (labelers generally use the 1.7 factor or a supplier-established one). dose_max_studied is capped at the UL; a 12-week randomized trial in children with autism used 2 mg/kg/day up to 50 mg/day under medical supervision, which is a drug regimen. Pair with vitamin B12.

Standardization: ≥97% calcium folinate (USP); supplied neat or as 1% dilutions

Source material: synthetic

As supplied: Often used as a 1% dilution on a carrier for microgram doses (0.1 mg powder per mcg); neat salt at mg doses. Supplier dependent.

Forms of Folate

FormTypical doseElement %FormatsPrice
Folinic Acid (Calcium Folinate)400–800 mcgCapsule, Tablet, Powder, Liquidhigh
Folic Acid400–800 mcgCapsule, Tablet, Powder, Gummy, Liquid, Softgellow
L-5-Methyltetrahydrofolate (L-5-MTHF)400–1,000 mcgCapsule, Tablet, Powder, Gummy, Liquid, Softgelhigh

Format suitability

  • Capsule
  • Powder
  • Tablet
  • Softgel
  • Gummy
  • Liquid

Light-sensitive; keep in opaque packaging. Rarely used in gummies.

Formulation notes

Leucovorin is used clinically to counter methotrexate and to potentiate fluorouracil, so any folinic acid product needs an interaction caution for those drugs. Pair with B12.

  • Has an upper limit
  • Notable drug interactions

Sources

  1. Folate — Health Professional Fact SheetNIH ODS
  2. Folinic acid improves verbal communication in children with autism and language impairment: a randomized double-blind placebo-controlled trial. (Frye RE, 2018)PubMed

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