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Bacillus subtilis DE111

Probioticspore-forming probioticBranded: DE111 (Deerland Probiotics & Enzymes (ADM))Moderate dose evidence

Also known as: DE111, B. subtilis DE111, Bacillus subtilis

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DE111 is a trademarked strain of Bacillus subtilis, a spore-forming soil and gut organism, supplied by Deerland (now part of ADM). Its randomized trials in healthy adults cover gastrointestinal and immune markers, blood lipids and endothelial function, and germination in the small intestine, at 1 to 5 billion CFU per day. Like other Bacillus spores it withstands heat, pressure, and low pH, so it is used in gummies, protein powders, and functional foods where vegetative probiotics would not survive. It is sold as a spore and germinates after ingestion.

Research summary

Spores germinate in the gut; studied for support of digestive enzyme activity, immune signaling, and competitive exclusion of undesirable organisms.

Gut & digestionImmuneHeart

Dosage

Daily amounts, of compound. CFU shown in billions.

Lowest studiedTypical rangeHighest studiedUpper limit
1 B CFU1–5 B CFU5 B CFU—

Amounts are in billions of CFU (enter 1 for 1 billion spores). Raw-material weight assumes a 100 billion CFU/g concentrate (10 mg per billion CFU); confirm the actual spore count per gram on the supplier CoA. Manufacturers add a 20–50% overage for processing losses; state CFU at manufacture or at expiry on the label. Trials dosed once daily with or without food.

Standardization: Strain DE111 spore preparation; CFU per gram on CoA

Source material: freeze-dried culture

As supplied: Assumes 100 billion CFU/g (10 mg per billion CFU); adjust to supplier CoA.

Forms of Bacillus

FormTypical doseElement %FormatsPrice
Bacillus subtilis DE1111–5 B CFU—Capsule, Powder, Gummymid
Bacillus coagulans1–2 B CFU—Capsule, Powder, Gummylow

Format suitability

  • Capsule
  • Powder
  • Tablet
  • Softgel
  • Gummy
  • Liquid

Spore-former; suitable for gummies, protein powders, and other heat-exposed formats. Budget overage for heat losses in gummies.

Formulation notes

Compatible with most matrices. Brand licensing may require label attribution; confirm with the supplier.

Sources

  1. NIH ODS — Probiotics Fact Sheet for Health ProfessionalsNIH ODS
  2. Examining the Gastrointestinal and Immunomodulatory Effects of the Novel Probiotic Bacillus subtilis DE111. (Freedman 2021)PubMed
  3. Bacillus subtilis DE111 intake may improve blood lipids and endothelial function in healthy adults. (Trotter 2020)PubMed

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