Bifidobacterium lactis

ProbioticbifidobacteriaStrong dose evidence

Also known as: B. lactis, Bifidobacterium animalis subsp. lactis, BB-12, HN019, Bi-07, Bl-04, DN-173 010

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Bifidobacterium animalis subsp. lactis, usually labeled B. lactis, is the workhorse bifidobacterium of the supplement industry because it is oxygen-tolerant and survives processing better than most Bifidobacterium species. Well-documented strains include HN019, which was tested in a dose-response trial of whole-gut transit time at roughly 1 and 17 billion CFU daily, and BB-12, widely used in dairy and infant products. It is used in regularity, digestive comfort, and immune formulas and pairs naturally with a Lactobacillus strain.

Research summary

Studied for effects on colonic transit, short-chain fatty acid production, and mucosal immune markers.

Dosage

Daily amounts, of compound. CFU shown in billions.

Lowest studiedTypical rangeHighest studiedUpper limit
1 B CFU1–10 B CFU17 B CFU

Amounts are in billions of CFU (enter 10 for 10 billion). Raw-material weight assumes a 100 billion CFU/g concentrate (10 mg per billion CFU); manufacturers add a 20–50% CFU overage at fill so the label claim holds through shelf life, and the label should state whether CFU are guaranteed at manufacture or at expiry. The HN019 transit-time trial compared about 1.8 and 17.2 billion CFU daily over 14 days.

Standardization: Strain-designated freeze-dried culture; CFU per gram on CoA

Source material: freeze-dried culture

As supplied: Assumes 100 billion CFU/g concentrate (10 mg per billion CFU); adjust to supplier CoA.

Forms of Bifidobacterium

FormTypical doseElement %FormatsPrice
Bifidobacterium lactis1–10 B CFUCapsule, Powdermid
Bifidobacterium longum1–10 B CFUCapsule, Powdermid
Bifidobacterium infantis (35624)1–10 B CFUCapsule, Powderhigh
Bifidobacterium bifidum1–5 B CFUCapsule, Powdermid

Format suitability

  • Capsule
  • Powder
  • Tablet
  • Softgel
  • Gummy
  • Liquid

Heat- and moisture-sensitive vegetative cells; capsule or dry powder only. Among the more processing-tolerant Bifidobacterium strains.

Formulation notes

Separate from antibiotic dosing by at least two hours. Keep water activity low in the finished product.

Sources

  1. NIH ODS — Probiotics Fact Sheet for Health ProfessionalsNIH ODS
  2. Dose-response effect of Bifidobacterium lactis HN019 on whole gut transit time and functional gastrointestinal symptoms in adults. (Waller 2011)PubMed
  3. Health benefits of Lactobacillus rhamnosus GG and Bifidobacterium animalis subspecies lactis BB-12 in children. (Szajewska 2020)PubMed

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